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Image Search Results
Journal: bioRxiv
Article Title: A Hypothalamic Circuit that Modulates Feeding and Parenting Behaviors
doi: 10.1101/2024.07.22.604437
Figure Lengend Snippet: a , food intake of lactating mice ( n = 14) and female controls ( n = 8). b , food intake during lactation (21 averaged days) vs. litter size ( n = 14 lactating mice). c-e , FED3 measurements showing hourly food intake of 21 averaged days per mouse ( c ), inter-pellet intervals ( d ), and meal sizes ( e ) during lactation period ( n lactating = 5 mice, n virgin = 6 mice). Pellets consumed within 60 s are part of one meal. f , schematic of 4 groups of mice for scRNA-seq created with BioRender. g-i , Agrp + ARC cluster: Venn diagrams of DEGs in fasted and lactating states ( g ), volcano plots of DEGs comparing fasted virgins vs. fed virgins (left) and fed lactating mothers vs. fed virgins (right) ( h ), and fold change correlation of shared DEGs in fasted and lactating states ( i ). j , firing rate of ARC NPY -hrGFP neurons (left, n virgin = 94 neurons, n lactating = 88 neurons) and mean firing rate per mouse (right, n = 6 mice per group). k , fiber photometry recordings of ARC AgRP -GCaMP6s in fed and fasted virgin ( n = 5) and lactating mice ( n = 6). l , mean ΔF/F after food presentation. Plots show mean ± s.e.m. Data were analyzed using simple linear regression ( b ), unpaired two-tailed t-test ( j ), or 2-way repeated-measures analysis of variance (RM-ANOVA) with Fisher’s Least Significant Difference (LSD) test ( l ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ns, non-significant. See Supplementary Table 1 for detailed statistical information.
Article Snippet: For channelrhodopsin-assisted circuit mapping, Brs3- T2A - FlpO;
Techniques: Two Tailed Test